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Preparation and Properties of Chloroplasts Depleted of Chloroplast Coupling Factor 1 by Sodium Bromide Treatment ¹

^a Department of Biology, Technicon-Israel Institute of Technology, Haifa, Israel

Chloroplasts were treated with 2 M sodium bromide. The resulting particles lost their ATPase activity and chloroplast coupling factor 1 subunits were detected in the supernatant by means of gel electrophoresis and specific antibodies. The chloroplast coupling factor 1 depleted particles show high rates of Hill reaction with pH optimum shifted toward lower pH. The sodium bromide treatment also abolished the light-induced proton uptake. In the presence of N-methylphenazonium methosulfate light-induced proton release, insensitive to uncouplers, was observed. Addition of dicyclohexylcarbodiimide reversed the light-induced pH changes to the normal proton uptake and increased the pH optimum of the Hill reaction.