This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (51) Citing Articles via Google Scholar Google Scholar Articles by Shepard, J. F. Articles by Totten, R. E. Search for Related Content PubMed PubMed Citation Articles by Shepard, J. F. Articles by Totten, R. E. Agricola Articles by Shepard, J. F. Articles by Totten, R. E.

Articles

Isolation and Regeneration of Tobacco Mesophyll Cell Protoplasts under Low Osmotic Conditions ¹

^a Department of Plant Pathology, Montana State University, Bozeman, Montana 59715

A method is described for the isolation of large numbers of tobacco (Nicotiana tabacum L. cv. Xanthi-nc) mesophyll cell protoplasts under relatively low external osmotic conditions. The procedure utilized 0.2 M sucrose as the primary osmoticum and a mixture of 0.5% macerozyme, 4% cellulase, and 2% polyvinylpyrrolidone, pH 5.4. The viability of resultant protoplasts was confirmed through regeneration of fertile plants. Plating and regeneration studies revealed, however, that qualitative and quantitative modifications in plating and differentiation media were necessary for protoplasts prepared in this manner. Over-all, the procedure was found to be a simplified alternative to those previously described for tobacco protoplast regeneration. In addition, the system should permit studies related to the influence of differing osmoticum levels on a variety of cell functions.