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Plant Physiology 55:734-737 (1975)
© 1975 American Society of Plant Biologists

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Articles

Ribonuclease in Senescing Morning Glory

Purification and Demonstration of de Novo Synthesis 1

B. Baumgartner, Hans Kende2 and P. Matile

a Department of General Botany, Swiss Federal Institute of Technology, Zurich, Switzerland

Isolated flower buds and flowers of Ipomoea tricolor went through the same stages of development as those attached to the plant. Ribonuclease activity increased sharply in both cases during the time of flower fading and aging. Affinity chromatography using guanosine diphosphate-Sepharose was employed for fast and efficient purification of ribonuclease. Flowers which were kept on D2O during the senescence phase incorporated deuterium into ribonuclease as shown by isopycnic density gradient centrifugation in CsCl, suggesting that ribonuclease was de novo synthesized during aging of the flower.

2 Permanent address: Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Mich. 48824.

1 This research was supported in part by the Jubiläumsfonds ETH 1930. H. K. was the recipient of a John Simon Guggenheim Memorial Foundation Fellowship.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1975 by the American Society of Plant Biologists