Plant Physiol.
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Plant Physiology 55:845-848 (1975)
© 1975 American Society of Plant Biologists

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Articles

14C2H4: Its Purification for Biological Studies 1

Elmo M. Beyer, Jr.

a Central Research Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898

A gas chromatographic technique is described for obtaining ultra high purity 14C2H4 for use in biological studies. 14C2H4 purchased from commercial sources contained readily detectable impurities including radioactive acetylene. Following purification on two different columns, no impurities were detected by high sensitivity gas chromatographic analysis. However, shortly thereafter impurities were detected as a result of radiation decomposition. Trapping and immediately regenerating ultra high purity 14C2H4 from dilute, filtered Hg (CIO4)2 solutions did not cause the formation of impurities, whereas additional impurities were formed when unpurified 14C2H4 was used. Impurities were also formed when ultra high purity 14C2H4 was stored in such solutions prior to its regeneration or when it was trapped and immediately regenerated from more concentrated Hg(CIO4)2 solutions.

1 Contribution No. 2234 from Central Research Department, Experimental Station, E. I. du Pont de Nemours & Co., Wilmington, Del. 19898.






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Copyright © 1975 by the American Society of Plant Biologists