Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 55:1038-1042 (1975)
© 1975 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Williams, J. P.
Right arrow Articles by Leung, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Williams, J. P.
Right arrow Articles by Leung, S.
Agricola
Right arrow Articles by Williams, J. P.
Right arrow Articles by Leung, S.
Articles

Galactolipid Synthesis in Vicia faba Leaves

I. Galactose, Glycerol, and Fatty Acid Labeling after 14CO2 Feeding 1

John P. Williams, Garry R. Watson, Mobashsher-Uddin Khan2 and Stephen Leung

a Department of Botany, University of Toronto, Toronto, Ontario, Canada

The galactose, glycerol, and fatty acids of mono- and digalactosyl diglycerides (MGDG and DGDG) have been separated and analyzed for 14C activity after 14CO2 feeding of Vicia faba leaf discs. Fully expanded and developing leaves were analyzed at time intervals following feeding during continuous illumination. In addition, fully expanded leaves were analyzed after similar times in complete darkness. In all cases, 14C was incorporated very rapidly into galactose, whereas glycerol and fatty acids were labeled much more slowly and over a longer period of time. The data are consistent with the galactosylation of a diglyceride to MGDG which is in turn galactosylated to DGDG. The data suggest that the formation of diglycerides suitable for galactosylation to MGDG is slow in comparison to the galactosylation process. It is also suggested that DGDG may be formed from more than one pool of MGDG. The complete analysis of the 14C incorporation into galactose appears to represent the only satisfactory method of comparing galactolipid synthesis by 14C incorporation. Estimates of comparative rates of synthesis of MGDG and DGDG have been made on this basis.

2 Present address: Department of Medicine (Neurology), Institute of Medical Science, University of Toronto.

1 This research was supported by a grant from the National Research Council of Canada to J. P. W.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1975 by the American Society of Plant Biologists