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Plant Physiology 56:72-77 (1975)
© 1975 American Society of Plant Biologists

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Articles

H2 Metabolism in Photosynthetic Organisms

I. Dark H2 Evolution and Uptake by Algae and Mosses 1

Ami Ben-Amotz, David L. Erbes2, Mary Ann Riederer-Henderson3, Dwight G. Peavey and Martin Gibbs

a Department of Biology, Brandeis University, Waltham, Massachusetts 02154 and Marine Biological Laboratories, Woods Hole, Massachusetts 02543

Dark H2 metabolism was studied in marine and fresh water red algae, the green alga, Chlamydomonas, and mosses. A time variable and temperature-sensitive anaerobic incubation was required prior to H2 evolution. H2 evolution was sensitive to disalicylidenepropanediamine. An immediate H2 uptake was observed in these algae. Immediate dark H2 uptake but no evolution was observed in the mosses.

A cell-free hydrogenase preparation was obtained from anaerobically adapted Chlamydomonas reinhardii by means of sonic oscillation. The hydrogenase was not sedimented at 100,000g. It catalyzed the reduction of methylene blue, p-benzoquinone, NAD, NADP, but not spinach ferredoxin. H2 evolution was noted with dithionite and with reduced methyl viologen as donors but not with reduced spinach ferredoxin. Similarly, hydrogenase activities were not affected by disalicylidenepropanediamine. The pH optima for H2 evolution and for H2 uptake were 7.2 and 7.5 to 9.5, respectively. Extracts prepared from the anaerobically adapted red alga, Chondrus crispus, and the moss, Leptobryum pyriforme, consumed but did not evolve H2. Uptake was slightly stimulated by methylene blue. It is proposed that red algae and mosses appear to metabolize H2 by a different pathway than Chlamydomonas.

2 Present address: Department of Biochemistry, University of Wisconsin, Madison, Wis. 53706.

3 Present address: Department of Biology, Rollins College, Winter Park, Fla. 32789.

1 This research was generously supported by Atomic Energy Commission Grant AT-11-1 3231 and National Science Foundation Grants GB29126X2 and BMS 73-00978.




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