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Plant Physiology 56:88-92 (1975)
© 1975 American Society of Plant Biologists

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Articles

Isolation and Translation of Plant Messenger RNA 1

Elaine M. Tobin2 and Attila O. Klein

a Department of Biology, Brandeis University, Waltham, Massachusetts 02154

A fraction of the RNA species isolated from Lemna gibba G-3 consists of molecules with attached sequences of polyadenylic acid. This polyadenylic acid-containing fraction, separated from total RNA by adsorption onto oligothymidylic acid-cellulose, was shown to be mRNA by its ability to serve as template in a cell-free translation system derived from wheat germ. The products of translation were characterized by electrophoresis. This method permitted the comparison of mRNA from plants grown under different light conditions. Such plants were shown to possess qualitative and quantitative differences in their mRNA complements.


2 Present address: Department of Biology, University of California, Los Angeles, Calif. 90024.

1 Research was supported by National Science Foundation Grant GB-36212. E.M.T. was the recipient of National Institutes of Health Traineeship from Training Grant T1-HD-22 and Institutional Grant RR-07044.







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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1975 by the American Society of Plant Biologists