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Occurrence of Pyruvate Orthophosphate Dikinase in the Succulent Plant, Kalanchoë daigremontiana Hamet. et. Perr. ¹

Watson M. Laetsch^b

^a Department of Agricultural Chemistry, School of Agriculture, Shizuoka University, Shizuoka 422, Japan, Department of Botany, University of California, Berkeley, California 94720

Pyruvate orthophosphate dikinase was detected from Kalanchoë daigremontiana Hamet. et. Perr., a succulent plant with crassulacean acid metabolism. Enzyme activity was similar to that of maize extracts. Two enzymes demonstrating pyruvate orthophosphate dikinase activity from K. daigremontiana and Zea mays were found to be partially identical from enzyme-inhibition and immunoprecipitin tests with maize enzyme antiserum. A time course study demonstrated that pyruvate orthophosphate dikinase activity in leaf extracts was dependent upon exposure of leaves to light.

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