Plant Physiol.
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Plant Physiology 56:617-621 (1975)
© 1975 American Society of Plant Biologists

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Articles

Ribosomal Changes during Induction of Cold Hardiness in Black Locust Seedlings 1

James A. Bixby and Gregory N. Brown

a School of Forestry, Fisheries, and Wildlife, University of Missouri, Columbia, Missouri 65201

Protein synthesis has been implicated in the cold-hardening process. Ribosomes from cold hardy and nonhardy black locust (Robinia pseudoacacia L.) seedlings were compared to determine if cold acclimation is related to alteration of ribosomal structure. Ribosomal structure, as indicated by thermal melting profiles, appears to be altered during induction of hardiness. Two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins indicates at least 17 proteins from hardy seedlings that are different from those of nonhardy seedlings. These different proteins may be partially responsible for the different thermal melting profiles observed.

1 Contribution from the Missouri Agricultural Experiment Station; Journal Series No. 7220.






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Copyright © 1975 by the American Society of Plant Biologists