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Plant Physiology 56:655-659 (1975)
© 1975 American Society of Plant Biologists

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Articles

Endogenous Gibberellins of Pine Pollen

II. Changes during Germination of Pinus attenuata, P. coulteri, and P. ponderosa Pollen 1

Aniela Kamienska and Richard P. Pharis

a Department of Biology, University of Calgary, Calgary, Alberta, T2N 1N4, Canada

The endogenous gibberellins (GAs) of pollen of Pinus attenuata, P. coulteri, and P. ponderosa were bioassayed at hour 0, 3, 15, 24, 48 and 72 of germination. Dormant pollen showed relatively high GA activity throughout the elution spectrum (i.e. ranging from relatively nonpolar to highly polar). The maximum GA activity was obtained at hour 15 in more polar regions and especially in the zone corresponding to GA3 (for P. attenuata estimated as 250 micrograms of GA3/kilogram pollen). It is probable that the "nonpolar" GAs present in high quantities in dormant pollen and in early stages of germination were converted to "more polar" GAs as germination progressed. The amount of all GAs decreased after hour 15 of germination and by hour 72 no GAs could be detected. Among the species tested P. attenuata showed the highest over-all GA activity.


1 This work was supported by a Canadian Forestry Service Extramural Grant, National Research Council Grant A-2585, and a Weyerhaeuser Grant to R.P.P.







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Copyright © 1975 by the American Society of Plant Biologists