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Studies with Detached Lupin Root Nodules in Culture

I. Maintenance and Induction of Acetylene Reduction Activity

Plant Physiology Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand

A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr^–1 mg^–1 fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acetylene reduction activity from 0.01 nmole or less to about 1 nmole hr^–1 mg^–1 fresh weight.