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Plant Physiology 56:786-790 (1975)
© 1975 American Society of Plant Biologists

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Articles

Purification of myo-Inositol 1-Phosphate Synthase from Rice Cell Culture by Affinity Chromatography 1

Edward A. Funkhouser2 and Frank A. Loewus3

a Division of Cell and Molecular Biology, State University of New York at Buffalo, Buffalo, New York 14214

myo-Inositol 1-phosphate synthase (EC 5.5.1.4) is the enzyme which catalyzes the synthesis of the precursor for the myo-inositol oxidation pathway. Rice callus grown in suspension culture provides a good source of plant enzyme. Use has been made of a noncompetitive inhibitor to prepare an affinity column for this enzyme. With this column, the enzyme from rice callus has been purified 1500-fold in a single step, about 9000-fold over-all, to a specific activity of 0.078 units per milligram of protein. This is an order of magnitude greater than previous purifications of the plant enzyme.

2 Present address: Forschungsstelle Vennesland der Max-Planck-Gesellschaft, 1 Berlin 33 (Dahlem), Harnackstrasse 23, Germany.

3 Present address: Department of Agricultural Chemistry, Washington State University, Pullman, WA 99163.

1 This work is supported by Grant GM-12422 from the National Institutes of Health.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1975 by the American Society of Plant Biologists