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Plant Physiology 56:821-825 (1975)
© 1975 American Society of Plant Biologists

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A Rapid Technique for the Estimation of Polynucleotide Adenylyltransferase and Ribonucleic Acid Polymerase in Plant Tissues 1

Trevor J. Walter and Rusty J. Mans

a Department of Biochemistry, University of Florida, Gainesville, Florida 32610

Nucleic acid-dependent polynucleotide adenylytransferase (EC 2.7.7.19) and ribonucleic acid polymerase (EC 2.7.7.6) have been partially purified from maize tissues (Zea mays L.) utilizing ammonium sulfate precipitation and batch diethylaminoethylcellulose chromatography. The technique is applicable to the simultaneous processing of up to eight samples of plant tissue and affords a rapid and reproducible means of assaying these two enzymes from small quantities of kernels or seedlings. The kinetic characteristics of the partially purified enzymes resemble those from more extensively purified preparations.

1 This investigation was supported in part by the United States Atomic Energy Commission, Division of Biology and Medicine, Report No. ORO-3982-35, and by the Florida Agricultural Experiment Station Journal Series No. 5854.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1975 by the American Society of Plant Biologists