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Plant Physiology 56:830-835 (1975)
© 1975 American Society of Plant Biologists

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Articles

Restoration of Organic Acid Accumulation in Sectioned Leaves of Bryophyllum tubiflorum Harv. 1,2

Thomas B. Kinraide and Mark J. Behan3

a Department of Biology, The Colorado College, Colorado Springs, Colorado 80903

When leaves of Bryophyllum tubiflorum were cut into transverse sections, and held at 20 C in the dark, the capacity to accumulate organic acid decreased with decreasing section thickness. In addition, the rate of respiration increased with decreasing section thickness and was unaffected by changes in O2 concentration above 5% or by the presence (1%) of CO2. It was concluded that O2 ventilation is not a controlling factor in respiration. Malonate (0.1 M) and fluoroacetate (0.01 M) restored the capacity of sectioned leaves to accumulate acid to normal levels and depressed respiration in 1-millimeter sections. Acid accumulation in 8-millimeter sections remained essentially constant at 20, 15, and 10 C, and was equal to that in unsectioned leaves, but accumulation in 2-millimeter sections rose to normal levels as the temperature fell to 10 C. Twenty-three additional metabolic inhibitors (none specific to the tricarboxylic acid cycle) were screened, and none promoted acid accumulation in sectioned leaves at 20 C. The results suggest that sectioning stimulates a respiratory sequence which includes the tricarboxylic acid cycle. This sequence in turn competes with the synthesis or accumulation of malic acid.

3 Present address: Department of Botany, University of Montana, Missoula, Mont. 59801.

1 This research was supported, in part, by McIntire-Stennis funds allocated to the School of Forestry, University of Montana, and by funds granted by the Research and Faculty Development Board of Colorado College.

2 Portions of this paper constitute a part of the Ph.D. thesis submitted at the University of Montana by T. B. K.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1975 by the American Society of Plant Biologists