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Articles

Isolation of Nucleoli and Localization of Ribonucleic Acid Polymerase I from Soybean Hypocotyl ¹

^a Department of Botany, University of Georgia, Athens, Georgia 30602

An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the -amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I.

¹ This research was supported by United States Public Health Service Research Grant CA-11624 from the National Cancer Institute to J. L. K.; T. J. G. was supported by a postdoctoral fellowship from the National Cancer Institute (CA01688-01).