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Plant Physiology 57:1-4 (1976)
© 1976 American Society of Plant Biologists

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Changes in Isoperoxidases during Cold Treatment of Dormant Pear Embryo 1,2

Kar-Ling Tao and Anwar A. Khan

a New York State Agricultural Experiment Station, Cornell University, Geneva, New York 14456

The number of isoperoxidases and the intensity of certain isozymes increased with increasing periods of stratification of pear (Pyrus communis cv. Bartlett) embryos. The presence of GA3 or kinetin during stratification enhanced the activity of certain isoperoxidases, and these enhancements were blocked in the presence of ABA which by itself had an inhibitory effect. Enhancement in isoperoxidases of pear embryos during stratification was inhibited by 6-methylpurine and cycloheximide; and in the presence of either of these two inhibitors, stratification failed to release the dormancy. Pear embryos germinated for 3 days showed changes in the pattern of isoperoxidases depending on the length of stratification.

1 This work was partially supported by grants from the Herman Frasch Foundation and the American Seed Research Foundation.

2 Approved by the Director of the New York State Agricultural Experiment Station as Journal Series Paper No. 2226.






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Copyright © 1976 by the American Society of Plant Biologists