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Plant Physiology 57:85-87 (1976)
© 1976 American Society of Plant Biologists

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Articles

In Vivo Conversion of 5-Oxoproline to Glutamate by Higher Plants 1

Mendel Mazelis2 and Helen M. Pratt

a Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, England

L-(U-14C)-5-oxoproline (pyrollidone carboxylic acid or pyroglutamic acid) was infiltrated into detached leaves of a number of species and incubated for 1 to 6 hours. In every case, conversion to labeled glutamate and glutamine was observed. The amount converted varied from 1 to 64% of the total label fed depending on the species. The ratio of glutamate-14C to glutamine-14C ranged from 5 in Vicia faba to 1 in sugar beet. This ratio could be affected by preinfiltrating various compounds before allowing the uptake of the 5-oxoproline. When L-methionine-DL-sulfoximine was prefed to sugar beet leaves, the glutamate-glutamine ratio increased from 1 to 10. Prior treatment of V. faba leaves with azaserine resulted in essentially only labeled glutamine being recovered. Preinfiltration with NaF or ATP gave similar results in that the glutamate-glutamine ratio was greatly decreased. The results are consistent with glutamate being produced from the 5-oxoproline and then being converted to glutamine.

2 Permanent address: Department of Food Science and Technology, University of California, Davis, Calif. 95616.

1 This work was supported by a grant to M.M. from the Underwood Fund of the Agricultural Research Council.




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N. Ohkama-Ohtsu, A. Oikawa, P. Zhao, C. Xiang, K. Saito, and D. J. Oliver
A {gamma}-Glutamyl Transpeptidase-Independent Pathway of Glutathione Catabolism to Glutamate via 5-Oxoproline in Arabidopsis
Plant Physiology, November 1, 2008; 148(3): 1603 - 1613.
[Abstract] [Full Text] [PDF]


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Copyright © 1976 by the American Society of Plant Biologists