This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (59) Citing Articles via Google Scholar Google Scholar Articles by Basha, S. M. M. Articles by Beevers, L. Search for Related Content PubMed PubMed Citation Articles by Basha, S. M. M. Articles by Beevers, L. Agricola Articles by Basha, S. M. M. Articles by Beevers, L.

Articles

Glycoprotein Metabolism in the Cotyledons of Pisum sativum during Development and Germination ¹

^a Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73069

The glycoprotein nature of legumin and vicilin, the reserve globulins in the cotyledons of Pisum sativum was studied. Legumin from mature seed was found to contain 1% neutral sugars (mannose and glucose) and 0.1% amino sugar (glucosamine), whereas vicilin contained 0.3% neutral sugar (mannose) and 0.2% amino sugar (glucosamine). On the basis of the incorporation of ¹⁴C-labeled glucosamine, it appeared that not all of the component subunits of the reserve proteins are glycosylated to the same extent. In addition, it has been established that glycosylation occurs after peptide synthesis. During seed development there was a change in neutral sugars and amino sugar ratio in vicilin. During germination, the neutral sugars and the amino sugar content of the glycoproteins declined. These findings are discussed in relation to the synthesis and degradation of the glycosyl component of the glycoproteins.

¹ This research was supported by National Science Foundation Grant GB 27318A. A preliminary report of this work was presented at a symposium on "The Biochemistry of Seed Germination" at the Los Angeles meeting of the American Chemical Society, 1974.