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Nonmetabolic Catalysis of ¹⁴C-Furfuryl Amino Purine (Kinetin) on Plant Surfaces, Glass, and Porcelain Ware

Kay Ryugo^b

Olav Messerschmidt^c

^a Department of Environmental Horticulture, University of California, Davis, California 95616, Department of Pomology, University of California, Davis, California 95616, Oroville, California 95965

Kinetin-8-¹⁴C degraded rapidly upon drying on living or inert surfaces. However, when care was exercised to avoid taking solutions to dryness during fractionation of plant extracts containing ¹⁴C-kinetin and before partitioning by thin layer chromatography, little degradation occurred. A procedure for 24-hour ethyl acetate partitioning, using a continuous liquid-liquid extractor, which permits nearly complete removal of kinetin from aqueous solutions, is herein described. High natural light intensities in the greenhouse or from fluorescent/incandescent sources greatly enhanced nonmetabolic degradation of kinetin on leaf (Bougainvillea) or glass surfaces, which indicated that this may be a confounding factor in analyzing metabolism of kinetin in plant tissues. One of the degradation products is probably adenine.