Plant Physiol. Drug Metab Dispos
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Plant Physiology 57:129-136 (1976)
© 1976 American Society of Plant Biologists

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Articles

Electrochemical Gradients and K+ and Cl– Fluxes in Excised Corn Roots 1,2

Robert F. Davis and Noe Higinbotham3

a Department of Botany, Rutgers University, Newark, New Jersey 07102

The compartmental analysis method was used to estimate the K+ and Cl– fluxes for cells of excised roots of Zea mays L. cv. Golden Bantam. When the measured fluxes are compared to those calculated with the Ussing-Teorell flux-ratio equation, an active inward transport of Cl– across the plasmalemma is indicated; the plasmalemma K+ fluxes are not far different from those predicted for passive diffusion, although an active inward transport cannot be precluded. Whether fluxes across the tonoplast are active or passive depends upon the vacuolar potential which is unknown. Assuming no electropotential gradient, the tracer flux ratios are fairly close to those predicted for passive movement. However, if the vacuole is positive by about 10 millivolts relative to the cytoplasm, the data suggest active inward transport for K+ and outward transport for Cl–.

Fluxes to the xylem exudate were found to be more accurately estimated from the specific radioactivity of the cytoplasm (symplasm) than from the external solution specific radioactivity. The electrochemical gradients for K+ and Cl– between the xylem vessels and the surrounding stelar parenchyma indicate active K+ and passive Cl– movement into the vessels. The data are interpreted as being in accord with radial transport through the symplast into living vessels.

3 Present address: Department of Botany, Washington State University, Pullman, Wash. 99163.

1 This investigation was supported by a grant from the Research Council of Rutgers University to R. F. D. and National Science Foundation Grants G-9560 and GB-5117X to N. H.

2 This paper is dedicated to Leon Bernstein in honor of his many contributions to plant physiology.






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Copyright © 1976 by the American Society of Plant Biologists