This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Verma, D. C. Articles by Loewus, F. A. Search for Related Content PubMed PubMed Citation Articles by Verma, D. C. Articles by Loewus, F. A. Agricola Articles by Verma, D. C. Articles by Loewus, F. A.

Articles

Effect of Benzyladenine, 2,4-Dichlorophenoxyacetic Acid, and D-Glucose on myo-Inositol Metabolism in Acer pseudoplatanus L. Cells Grown in Suspension Culture ¹

^a Division of Cell and Molecular Biology, State University of New York at Buffalo, Buffalo, New York 14214

Suspension cultures of Acer pseudoplatanus L. cells grown for 15 days in medium (T. Murashige and F. Skoog. 1962. Physiol. Plant. 15: 473-497) contained 3% sucrose, 1 mg/l 6-benzylaminopurine (BA), and 0.1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), referred to here as normal media, removed newly added myo-inositol-2-³H up to 100 mg/l in 24 hours and utilized up to 20% of this cyclitol for pectin biosynthesis. When the BA content of the growth medium was raised 10-fold, uptake of myo-inositol was drastically reduced and very little was available for pectin biosynthesis. Neither cell growth as measured by packed cell volume or by dry weight, nor monomer composition of pectic polysaccharides was affected by the increased level of cytokinin. Increasing, 2,4-D 10-fold instead of BA had little or no effect on myo-inositol uptake, although it did reduce the amount of myo-inositol utilized for pectin biosynthesis. Cells grown 15 days in normal media failed to remove added myo-inositol if 3% D-glucose was included. The net result was similar to that found in cells grown in the high BA condition. If a trace amount of D-galactose-1-¹⁴C was supplied to cells after 15 days of growth in normal, high BA, or high 2,4-D media, there was no significant variation in uptake and utilization of label among the three growth conditions.

³ Present address: Department of Agricultural Chemistry, Washington State University, Pullman, Wash. 99163.

¹ This investigation was supported by National Institutes of Health Grant GM-12422 from the National Institute of General Medical Sciences. This research was taken in part from a thesis submitted by D. C. V. in partial fulfillment of his requirements for the Ph.D., SUNY/Buffalo, 1975. A preliminary report of this work has appeared elsewhere (29).