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Plant Physiology 57:353-357 (1976)
© 1976 American Society of Plant Biologists

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Articles

Boron Uptake by Excised Barley Roots

I. Uptake into the Free Space 1,2

John E. Bowena

Per Nissenb

a Department of Botany, Hawaii Agricultural Experiment Station, University of Hawaii, Hilo, Hawaii 96720, b Botanical Laboratory, University of Bergen, 5014 Bergen, Norway

At 2 C, all boron accumulated by excised barley roots (Hordeum vulgare L. cv. Herta) remains in the free space; i.e. active uptake is nil at this temperature. Three component fractions of free space B were apparent: (a) a surface contaminant film of B on blotted roots, (b) water free space B, and (c) B reversibly bound in the cell walls. A stoichiometric release of H+ from the roots in the presence of B indicated that B was bound by borate complexes with polysaccharides in the cell walls. Polysaccharide-borate complexes are much less stable than those of monosaccharides, and the bound B fraction could be readily removed by rinsing the roots in the presence of a monomeric polyol possessing the necessary cis-diol configuration. Cell wall material separated from excised barley roots had a B binding capacity 66% greater than that of intact roots.

A 30-minute rinse in distilled H2O or 0.5 mM CaSO4 was required to remove all cell wall-bound B from the roots after a 30-minute uptake period. Thus, although B in the contaminant surface film and the water free space is rinsed from the roots within 10 minutes, a 30-minute rinse is essential if all reversibly accumulated B is to be removed from the free space.

1 This work was supported by a grant to the senior author from the Norwegian Council for the Sciences and Humanities.

2 Journal Series No. 1894 of the Hawaii Agricultural Experiment Station.






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Copyright © 1976 by the American Society of Plant Biologists