This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (66) Citing Articles via Google Scholar Google Scholar Articles by Gonzalez, E. Articles by Beevers, H. Search for Related Content PubMed PubMed Citation Articles by Gonzalez, E. Articles by Beevers, H. Agricola Articles by Gonzalez, E. Articles by Beevers, H.

Articles

Role of the Endoplasmic Reticulum in Glyoxysome Formation in Castor Bean Endosperm ¹

^a Thimann Laboratories, University of California, Santa Cruz, California 95064

Homogenates of the endosperm of castor bean (Ricinus communis var. Hale) were prepared at intervals during germination and fractionated on sucrose gradients. Early in germination when glyoxysomes were being produced, a substantial proportion (50%) of the activities of malate synthetase and citrate synthetase was recovered in the membranes of the endoplasmic reticulum (mean density 1.12 grams per cubic centimeter). This proportion declined to less than 10% at 4 days when the glyoxysomes were fully developed.

Gradient fractions challenged by antiglyoxysome-protein antiserum in double immunodiffusion assay revealed strong antigenic response in the endoplasmic reticulum membranes. The results support the view advanced earlier that glyoxysomes are derived directly from the endoplasmic reticulum.

¹ This work was supported by the Atomic Energy Commission (Contract AT-1 04/3). E.G. held a National Institute of Health Postdoctoral Fellowship (1FO2 GM 55311-01).