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Plant Physiology 57:424-429 (1976)
© 1976 American Society of Plant Biologists

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Articles

Influence of the Nutrient Medium on the Recovery of Dividing Cells from Tobacco Protoplasts 1,2

Hirofumi Uchimiya and Toshio Murashige

a Department of Plant Sciences, University of California, Riverside, California 92502

Systematic tests resulted in a nutrient solution containing the following, in milligrams per liter, for the culture of protoplasts isolated from Nicotiana tabacum L. callus cells: Murashige and Skoog salts (T. Murashige and F. Skoog, 1962. Physiol. Plant. 15: 473-497); sucrose, 15,000; mannitol, 110,000; {alpha}-naphthaleneacetic acid, 0.6; kinetin, 0-0.1; thiamine·HCl, 10; pyridoxine·HCl, 10; nicotinic acid, 5; myo-inositol, 100; and glycine, 2. In this medium, regeneration of cell wall has been observed in 85% and resumption of cell division among 35% of the protoplast isolates.

1 This research was supported in part by an Elvenia J. Slossan Fellowship in Ornamental Horticulture awarded to T.M.

2 This paper represents part of the Ph.D. dissertation of H. U.






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Copyright © 1976 by the American Society of Plant Biologists