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Plant Physiology 57:572-576 (1976)
© 1976 American Society of Plant Biologists

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Comparative Studies on the Induction and Inactivation of Nitrate Reductase in Corn Roots and Leaves 1

Muhammad Aslam2 and Ann Oaks3

a Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1

A comparison of induction and inactivation of nitrate reductase and two of its component activities, namely FMNH2-nitrate reductase and NO3–-induced NADH-cytochrome c reductase, was made in roots and leaves of corn (Zea mays L. var. W64A x 182E). The three activities were induced in parallel in both tissues when NO3– was supplied. WO4= suppressed the induction of NADH- and FMNH2-nitrate reductase activities in root tips and leaves. The NO3–-induced NADH-cytochrome c reductase activity showed a normal increase in roots treated with WO4=. In leaves, on the other hand, there was a marked superinduction of the NO3–-induced NADH-cytochrome c reductase in the presence of WO4=.

The half-life values of NADH-nitrate reductase and FMNH2-nitrate reductase measured by removing NO3– and adding WO4= to the medium, were 4 hours in root tips and 6 hours in excised leaves. Addition of NO3– in the induction medium together with WO4= gave partial protection of NADH-nitrate reductase and FMNH2-nitrate reductase activities in both root tips and leaves with a t0.5 of 6 and 8 hours, respectively. NO3– also reduced the loss of nitrate reductase activity from mature root sections. In the presence of cycloheximide, both NADH-nitrate reductase and NO3–-induced NADH-cytochrome c reductase activities were lost at similar rates in root tips. NO3– protected the loss of NO3–-induced NADH-cytochrome c reductase to the same extent as that of NADH-nitrate reductase.

2 Present address: Department of Crop Science, University of Guelph; Guelph, Ontario 2NG 1 W3.

3 To whom reprint requests should be addressed.

1 This work was supported by Grant A-2818 from the National Research Council of Canada.






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Copyright © 1976 by the American Society of Plant Biologists