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Plant Physiology 57:580-582 (1976)
© 1976 American Society of Plant Biologists

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Articles

Isotope Discrimination by Ribulose 1,5-Diphosphate Carboxylase

No Effect of Temperature or HCO3 Concentration 1

John T. Christellera and William A. Lainga

John H. Troughtonb

a Plant Physiology Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand, b Physics and Engineering Laboratory, Department of Scientific and Industrial Research, Lower Hutt, New Zealand

Carbon 13 isotope discrimination by ribulose 1,5-diphosphate carboxylase from soybean (Glycine max [Merr.] cv. Amsoy) was studied as a function of temperature, bicarbonate concentration, and pH. None of these factors affected the degree of discrimination against 13C. The average {delta}13C was –28.3%, a value close to that found for whole C3 plants. The zero temperature response observed here with ribulose 1,5-diphosphate carboxylase corroborates data from whole plants. The lack of effect of bicarbonate concentration on discrimination is consistent with both current theories of alternate forms of carboxylase.


1 Dedicated to the memory of J. W. Lyttleton.




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Copyright © 1976 by the American Society of Plant Biologists