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Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L. ¹

^a Cabot Foundation, Harvard University, Petersham, Massachusetts 01366

Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea (Pisum sativum L., cv. Little Marvel). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems. Smaller activities of zeatin ribotide, isopentenyladenine and its riboside were also detected. Cytokinin activity gradually decreased with the cultivation period, but no qualitative change in the active compounds was found.

¹ This research was supported in part by the Maria Moors Cabot Foundation for Botanical Research of Harvard University and by Research Grant BMS 74-20563 of the National Science Foundation.