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Plant Physiology 57:645-646 (1976)
© 1976 American Society of Plant Biologists

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Aliphatic Chains of Esterified Lipids in Isolated Eyespots of Euglena gracilis var. bacillaris1

Lula L. Hilenski, Patricia L. Walne and Fred Snyder

Department of Botany, University of Tennessee, Knoxville, Tennessee 37916, Medical and Health Sciences Division, Oak Ridge Associated Universities, Oak Ridge, Tennessee 37830

Isolated eyespot granules of Euglena gracilis Klebs var. bacillaris Pringsheim contained approximately 6% lipids (based on protein). Separation of the lipid extracts by thin layer chromatography revealed four major fractions: wax esters, triacylglycerols, free fatty acids, and phospholipids. Methanolysis of each fraction yielded between 27 and 29 different fatty acids ranging from 12:0 to 22:6. Acetates of the fatty alcohols of the wax fraction consisted of 11:0 to 18:0 carbon chains, with 14:0 being the major component; unsaturated alcohols were not detected.

1 This investigation was supported in part by National Science Foundation Grant BMS 69-00229 A 04, in part by a University of Tennessee Faculty Research Grant, and in part by senior Fulbright-Hays and A.A.U.W. Postdoctoral Research Fellowships to P.L.W., and in part by the Energy Research and Development Administration. Contribution No. 459 from the Department of Botany, University of Tennessee, Knoxville.




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Copyright © 1976 by the American Society of Plant Biologists