Plant Physiol.
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Plant Physiology 57:720-723 (1976)
© 1976 American Society of Plant Biologists

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Articles

Extractant Influence on the Relationship between Extractable Proteins and Cold Tolerance of Alfalfa 1,2

Wade F. Faw3, Sao C. Shih4 and Gerald A. Jung5

a Division of Plant Sciences-Agronomy and Genetics, West Virginia University, Morgantown, West Virginia 26506

The influence of ionic composition and pH of extractant on the relationship between the extracted proteins and the cold tolerance of Vernal and Arizona Common alfalfa (Medicago sativa L.) was examined. Five environments were used to induce different tolerance levels. The quantity of protein extracted from plants was influenced by the hardening environment, cultivar, and ionic composition and pH of 29 extractants. Extractants with a pH below 6 generally extracted less protein.

The measured cold tolerance of the plants was correlated with the quantity of protein detected in many of the 14 regions of the electrophoresis gel columns regardless of extractant but was most closely associated with the protein in either region 7 or 8 with nine of ten extractants.

The magnitudes of cultivar and hardening effects on quantities of protein detected on various column regions were influenced by choice of extractant. This suggests that proper extractant selection may be vital for study of isozymes by electrophoresis.

3 Present address: Plant and Soil Science Department, Tennessee Technological University, Cookeville, Tenn. 38501.

4 Present address: Taiwan Sugar Experiment Station, Tainan, Taiwan.

5 Present address: United States Regional Pasture Research Laboratory, University Park, Pa. 16802.

1 Research was supported in part by National Science Foundation Grant GB-12618.

2 Published with the approval of the Director of the West Virginia University Agricultural Experiment Station as Scientific Paper No. 1358. This study includes data from a dissertation submitted by W.F.F. to the Graduate School of West Virginia University in partial fulfillment of the requirements for the Ph.D. degree.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1976 by the American Society of Plant Biologists