Plant Physiology 57:751-759 (1976)
© 1976 American Society of Plant Biologists
Articles
Host-Pathogen Interactions
IX. Quantitative Assays of Elicitor Activity and Characterization of the Elicitor Present in the Extracellular Medium of Cultures of Phytophthora megasperma var. sojae1
Arthur R. Ayers2,
Jürgen Ebel3,
Frederick Finelli,
Nathan Berger and
Peter Albersheim4
a Department of Molecular, Cellular and Developmental Biology and Department of Chemistry, University of Colorado, Boulder, Colorado 80302
Resistance of soybean (Glycine max L.) seedlings to Phytophthora megasperma var. sojae (Pms) is in part due to the accumulation in infected tissue of a compound which is toxic to Pms. The accumulation of this compound, a phytoalexin called glyceollin, is triggered by infection, but it can also be triggered by molecules, "elicitors," present in cultures of Pms. The ability of the Pms elicitor to stimulate phytoalexin accumulation in soybean tissues has been used as the basis for biological assays of elicitor activity. Two bioassays were developed and characterized in this study of the Pms elicitor. These bioassays use the cotyledons and the hypocotyls of soybean seedlings. The cotyledon assay was used to characterize the extracellular Pms elicitor. This elicitor was isolated from Pms cultures and purified by ion exchange and molecular sieving chromatography. The extracellular Pms elicitor was determined to be a predominantly 3-linked glucan, which is similar in composition and structure to a polysaccharide component of Pms mycelial walls.
2 Present address: Swedish Forest Products Research Laboratory, Box 5604, S-114 86, Stockholm, Sweden.
3 Present address: Biologie II der Universität, D-78 Freiburg, West Germany.
4 To whom reprint requests should be addressed at the Department of Chemistry.
1 Research was supported by the Energy Research and Development Administration E(11-1)-1426, the Herman Frasch Foundation, New York City, a University of Colorado Faculty Fellowship to P. A. and a Deutsche Forschungsgemeinschaft Fellowship to J. E.
This article has been cited by other articles:
|
|
|
|
 
V. V. Lozovaya, A. V. Lygin, S. Li, G. L. Hartman, and J. M. Widholm
Biochemical Response of Soybean Roots to Fusarium solani f. sp. glycines Infection
Crop Sci.,
May 1, 2004;
44(3):
819 - 826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. V. Modolo, F. Q. Cunha, M. R. Braga, and I. Salgado
Nitric Oxide Synthase-Mediated Phytoalexin Accumulation in Soybean Cotyledons in Response to the Diaporthe phaseolorum f. sp. meridionalis Elicitor
Plant Physiology,
November 1, 2002;
130(3):
1288 - 1297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. C. Rose, K.-S. Ham, A. G. Darvill, and P. Albersheim
Molecular Cloning and Characterization of Glucanase Inhibitor Proteins: Coevolution of a Counterdefense Mechanism by Plant Pathogens
PLANT CELL,
June 1, 2002;
14(6):
1329 - 1345.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Klarzynski, B. Plesse, J.-M. Joubert, J.-C. Yvin, M. Kopp, B. Kloareg, and B. Fritig
Linear beta -1,3 Glucans Are Elicitors of Defense Responses in Tobacco
Plant Physiology,
November 1, 2000;
124(3):
1027 - 1038.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. A. Bhagwat, A. Mithöfer, P. E. Pfeffer, C. Kraus, N. Spickers, A. Hotchkiss, J. Ebel, and D. L. Keister
Further Studies of the Role of Cyclic beta -Glucans in Symbiosis. An ndvC Mutant of Bradyrhizobium japonicum Synthesizes Cyclodecakis-(1right-arrow3)-beta -Glucosyl
Plant Physiology,
March 1, 1999;
119(3):
1057 - 1064.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Umemoto, M. Kakitani, A. Iwamatsu, M. Yoshikawa, N. Yamaoka, and I. Ishida
The structure and function of a soybean beta -glucan-elicitor-binding protein
PNAS,
February 4, 1997;
94(3):
1029 - 1034.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kita, C. M. Boyd, M. R. Garrett, F. Jurnak, and N. T. Keen
Differential Effect of Site-directed Mutations in pelC on Pectate Lyase Activity, Plant Tissue Maceration, and Elicitor Activity
J. Biol. Chem.,
October 25, 1996;
271(43):
26529 - 26535.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
