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Plant Physiology 57:802-805 (1976)
© 1976 American Society of Plant Biologists

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Articles

Carbohydrate-limited Growth Kinetics of Tobacco (Nicotiana rustica L.) Callus 1

Warren F. Hunt2 and Robert S. Loomis

a Department of Agronomy and Range Science, University of California, Davis, California 95616

Logistic curves were fitted to sigmoidal growth data obtained from tobacco (Nicotiana rustica L.) callus grown on media prepared with 0.1, 0.03, 0.01, and 0.003 M sucrose. Analysis of the growth curves indicated that final yields and specific growth rates were influenced by the initial sucrose concentration. Growth yields from the four treatments were similar (0.61 ± 0.04 gram dry tissue per gram sucrose supplied). Initial specific growth rates exhibited a Michaelis-Menten dependency on initial sucrose concentration such that the Vmax = 0.18 g g–1 day–1 and Km = 0.0037 M sucrose.

2 Present address: Grasslands Division, DSIR, Private Bag, Palmerston North, New Zealand.

1 This work was supported by Grant GB 18541 from the National Science Foundation, and by the Department of Scientific and Industrial Research, Palmerston North, New Zealand.






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Copyright © 1976 by the American Society of Plant Biologists