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Plant Physiology 57:850-854 (1976)
© 1976 American Society of Plant Biologists

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Articles

Auxin Biogenesis

Subcellular Compartmentation of Indoleacetaldehyde Reductases in Cucumber Seedlings 1

Peter J. Bower2, Hugh M. Brown and William K. Purves

a Biochemistry and Biophysics Section, Biological Sciences Group, University of Connecticut, Storrs, Connecticut 06268

Subcellular fractionation of cucumber (Cucumis sativus L.) seedlings was achieved, and two of the enzymes in the auxin biosynthetic pathway were localized. NADH-specific indoleacetaldehyde reductase activity was observed only in the cytosol fractions obtained from separated hypocotyl and cotyledon tissue. In contrast, a portion of the NADPH-specific indoleacetaldehyde reductase activity was associated with a microsomal fraction derived from these tissues. The NADPH-specific indoleacetaldehyde reductase was consistently found to be more firmly associated with the microsomal fraction derived from hypocotyls than with that from the cotyledons. These results indicate a division of the terminal steps of auxin biogenesis into at least two subcellular compartments.

2 University Scholar, Undergraduate Honors Program, University of Connecticut.

1 This work was supported by the University of Connecticut Research Foundation and by National Science Foundation Grant GB-40556 to W.K.P.






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Copyright © 1976 by the American Society of Plant Biologists