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Plant Physiology 57:894-897 (1976)
© 1976 American Society of Plant Biologists

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An Improved Cytokinin Bioassay Using Cultured Soybean Hypocotyl Sections 1

Peter J. Manos and Jonathan Goldthwaite

a Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167

This paper describes a modified soybean (Glycine max) tissue culture bioassay for cytokinins. Soybean hypocotyls were grown under sterile conditions and sliced into 1-mm sections. Sections were cultured for 5,9,13, or 22 days on a callus medium with zeatin or other cytokinins. The fresh weight of sections increased with the cytokinin concentration from 0.0005 to 1 µM zeatin; 2-fold concentration differences were readily distinguishable at 9 days. The assay should prove to have several advantages over the conventional soybean callus bioassay including convenience, lower variability between tissue samples, and improved resolution. Its specificity is comparable to that of the soybean callus bioassay.

1 This work was supported in part by a National Institutes of Health Grant HD 09280. P. J. M. was supported by National Institutes of Health Training Grant To 1 GM 00036 while a graduate student in the Department of Biology at Harvard University.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1976 by the American Society of Plant Biologists