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Starch Degradation in Isolated Spinach Chloroplasts ¹

^a Institute for Photobiology of Cells and Organelles, Brandeis University, Waltham, Massachusetts 02154

A method for loading isolated intact spinach (Spinacia oleracea L.) chloroplasts with ¹⁴C-starch is described. These intact chloroplasts were incubated aerobically in the dark for 30 minutes. Radioactivity in starch declined and glyceric acid 3-phosphate and maltose were the major radioactive products. It is proposed that starch is degraded within the chloroplast to glyceric acid 3-phosphate and to maltose.

³ Present address: Department of Biochemistry and Biophysics, University of California, Davis, Calif. 95616.

¹ This research was supported by National Science Foundation Grant BMS71-00978.

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