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Plant Physiology 58:68-70 (1976)
© 1976 American Society of Plant Biologists

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Articles

Endogenous Gibberellins of Pine Pollen

III. Conversion of 1,2-[3H]GA4 to Gibberellins A1 and A34 in Germinating Pollen of Pinus attenuata Lemm. 1

Aniela Kamienska, Richard C. Durley2 and Richard P. Pharis

a Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4

Gibberellins A1 and A34 (possibly A2) were found as products of metabolism of 1,2-[3H]GA4 during germination of Pinus attenuata pollen. The conversion from GA4 to GA1 and GA34 occurred as hydroxylations at atoms C-13 and C-2 of the ent-gibberellane skeleton, respectively. Percentage interconversion of the GA4 absorbed was in the range of 0.15 to 0.43% for GA1 and 1.54 to 3.22% for GA34. Identifications were made on a gas-liquid chromatograph with radioactive monitoring by comparison with standards.

2 Present address: Crop Science Department, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N OWO.

1 This work was supported by a Canadian Forestry Service Extramural Grant, NRC Grant No. A-2585, and a Weyerhaeuser Grant to R. P. P.






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Copyright © 1976 by the American Society of Plant Biologists