Plant Physiol. Drug Metab Dispos
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Plant Physiology 58:87-90 (1976)
© 1976 American Society of Plant Biologists

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Articles

Fractionation and Analysis of Polypeptides of Euglena gracilis Chloroplasts 1

Aurea C. Vasconcelos, Leticia R. Mendiola-Morgenthaler, Gary L. Floyd2 and Jeffrey L. Salisbury2

a Department of Botany and Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey 08903

Intact Euglena gracilis chloroplasts, purified on gradients of silica sol, were lysed osmotically and fractionated by centrifugation on discontinuous gradients of sucrose into their soluble, envelope membrane, and thylakoid membrane components. The proteins of the different subchloroplast fractions, as well as those of whole chloroplasts, were analyzed by electrophoresis on sodium dodecyl sulfate polyacrylamide gels. The polypeptide profile of each fraction was distinctive and was in general similar to the profile obtained for analogous fractions of the chloroplasts of higher plants.

The envelope membranes were separated into two fractions in the gradients according to their banding densities. Electron micrographs showed that the light envelope fraction consisted mostly of single-membrane vesicles, whereas the heavy envelope fraction consisted of multiple layers of folded membranes. Both envelope fractions were ultrastructurally distinct from the thylakoid membranes.

2 Present address: Department of Botany, School of Biological Sciences, Ohio State University. Columbus. Ohio 43210.

1 This work was supported in part by United States Public Health Service Grant HD-05602 and the Charles and Johanna Busch Memorial Fund to C. A. Price, and by a grant from the Rutgers Research Council to A. C. V.






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Copyright © 1976 by the American Society of Plant Biologists