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Articles

Analysis of Native Gibberellins in the Internode, Nodes, Leaves, and Inflorescence of Developing Avena Plants ¹

Richard P. Pharis^b, Richard C. Durley^b,3 and William Morf^b

^a Department of Botany, Division of Biological Sciences, University of Michigan, Ann Arbor, Michigan 48109, ^b Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4, Canada

The native gibberellins (GAs) of various organs of the Avena plant were analyzed by bioassay and gas chromatography-mass spectrometry (GC-MS) after silicic acid partition column chromatography. The major GA of the inflorescence was identified as GA₃ by GC-MS, and this GA also forms the major component of the nodes, p-1 internode, and roots as determined by GLC or chromatography/bioassay. The inflorescence and nodes are the major sources of native GAs, the last two leaves, internode, and roots having significantly lower amounts of GA-like substances. In the internode, less polar GAs predominated at the lag stage of development, whereas by the log and plateau stages, the more polar GAs increased significantly.

Since less polar GAs are early in oxidative interconversion sequences, this finding indicates sequential conversion to more polar and probably more active GAs, during log phase growth of the p-1 internode.

³ Present address: Crop Science Department, University of Saskatchewan, Saskatoon S7N OWO, Canada.

¹ This research was supported by Grant BMS 75-16359 from the National Science Foundation to P. B. K. and Grant A-2585 from the National Research Council of Canada to R. P. P.

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