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Plant Physiology 58:299-303 (1976)
© 1976 American Society of Plant Biologists

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Articles

Indole-3-acetic Acid in Douglas Fir

Analysis by Gas-Liquid Chromatography and Mass Spectrometry

David R. Deyoe1 and Joe B. Zaerr

a Forest Research Laboratory, School of Forestry, Oregon State University, Corvallis, Oregon 97331

We sought evidence for the occurrence and seasonal variation of indole-3-acetic acid (IAA) in shoots of Douglas fir (Pseudotsuga menziesii [Mirb.] Franco).

Collections obtained in December and June were extracted with methanol and diethyl ether. Extracts were purified by solvent partitioning and with Sephadex LH-20. Qualitative and quantitative information was acquired by gas-liquid chromatography of methyl, trimethylsilyl, or both derivatives of plant extract components. Analysis was performed with polar (XE-60) and moderately polar (Hi-Eff-8-BP) stationary phases. Results from three collections demonstrated that IAA does occur in Douglas fir and that amounts vary seasonally. Mass analysis of the proposed endogenous IAA peak from two representative extracts supported gas-liquid chromatography data and established the presence of IAA in Douglas fir.

1 This report is based on a portion of the research submitted in partial fulfillment of requirements for a Master of Science degree. Present address: School of Forestry, Fisheries and Wildlife, University of Missouri, Columbia, Missouri 65201.






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Copyright © 1976 by the American Society of Plant Biologists