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Plant Physiology 58:324-330 (1976)
© 1976 American Society of Plant Biologists

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Articles

Association of Latent Cellulase Activity with Plasma Membranes from Kidney Bean Abscission Zones 1

Don E. Koehler, Robert T. Leonard, William J. Vanderwoude, Arthur E. Linkins2 and Lowell N. Lewis

a Departments of Plant Sciences and Biology, University of California, Riverside, California 92502

Membranes isolated from abscission zones of Phaseolus vulgaris L., cv. Red Kidney, contained cellulase activity. This particulate activity was enhanced 10- to 20-fold by treatment with Triton X-100. Sucrose density gradient analyses of cell fractions showed that the membranes with which cellulase was associated had a peak equilibrium density of 1.16 to 1.17 g/cm3 which coincided with that of ion-activated ATPase, a marker for plasma membranes. The membrane fraction having the highest cellulase activity also contained a high proportion of plasma membranes as shown by electron microscopy of sucrose density gradient fractions after staining by periodic acid-chromic acid-phosphotungstic acid. It was concluded that the particulate cellulase was associated with the plasma membrane.

2 Present address: Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Va. 24061.

1 This research was supported by Grant No. BMS74-19986 from the National Science Foundation.




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D. A. Brummell, C. Catala, C. C. Lashbrook, and A. B. Bennett
A membrane-anchored E-type endo-1,4-beta -glucanase is localized on Golgi and plasma membranes of higher plants
PNAS, April 29, 1997; 94(9): 4794 - 4799.
[Abstract] [Full Text] [PDF]


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