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Plant Physiology 58:666-669 (1976) © 1976 American Society of Plant Biologists Metabolism of Indole-3-Acetic AcidIII. Identification of Metabolites Isolated from Crown Gall Callus Tissue 1a Departments of Entomology and Biology, Pesticide Research Laboratory and Graduate Study Center, The Pennsylvania State University, University Park, Pennsylvania 16802
The metabolism of labeled indole-3-acetic acid (IAA-2-14C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were aspartic and glutamic acid (minor ones) as well as glycine, alanine, and valine (major ones). The last four are being reported for the first time as metabolites of IAA. These conjugates were identified through their chromatographic properties, hydrolysis products, and their mass spectra. The possible significance of these amino acid conjugates is discussed.
2 Present Address: Union Carbide Corp., Technical Center, South Charleston, West Virginia 25303. 1 This work has been authorized for publication as Paper No. 5029 in the Journal Series of The Pennsylvania Agricultural Experiment Station. Supported in part by the Northeastern Regional: Research Project NE-53 and Regional Research Funds. This article has been cited by other articles:
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