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Articles

Cytokinins in Corynebacterium fascians Cultures

Isolation and Identification of 6-(4-Hydroxy-3-methyl-cis-2-butenylamino)-2-methylthiopurine ¹

Douglas L. Cole^b,4 and Nelson J. Leonard^b,3

^a Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wisconsin 53706, ^b Roger Adams Laboratory, School of Chemical Sciences, University of Illinois, Urbana, Illinois 61801

In addition to the four cytokinins, 6-(3-methyl-2-butenylamino)purine, 6-methylaminopurine and the cis and trans isomers of 6-(4-hydroxy-3-methyl-2-butenylamino)purine, reported earlier from our laboratories, three cytokinin-active fractions have been obtained from the aqueous medium of 6-day-old Corynebacterium fascians cultures. One of these has been identified as 6-(4-hydroxy-3-methyl-cis-2-butenylamino)-2-methylthiopurine (2-methylthio-cis-zeatin, c-ms²io⁶ Ade).

The elution volumes of the other two fractions correspond to those of authentic 6-(4-hydroxy-3-methyl-2-butenylamino)-9--D-ribofuranosyl-purine and 6-(3-methyl-2-butenylamino)-9--D-ribofuranosylpurine, indicating the presence of trace amounts of these two ribonucleosides.

³ To whom requests for reprints should be sent.

⁴ Present address: Merck Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065.

¹ This work was supported at the University of Wisconsin by National Science Foundation Research Grant BMS72-02226 to F. S. and the University of Illinois by Research Grant GM-05829 from the United States Public Health Service National Institutes of Health to N. J. L.

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