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Influence of Carbohydrates on Quantitative Aspects of Growth and Embryo Formation in Wild Carrot Suspension Cultures ^1,2

^a W. Alton Jones Cell Science Center, Old Barn Road, Lake Placid, New York 12946

Wild carrot (Daucus carota L.) cell suspensions were grown on a mineral salt medium supplemented with 10 mMmyoinositol in the presence and absence of 2.25 µM 2,4-dichlorophenoxyacetic acid (2,4-D), and a variety of carbon sources.

The data obtained on growth and embryo number in the absence of 2,4-D show that wild carrot suspensions were able to utilize sucrose, glucose, fructose, galactose, mannose, maltose, raffinose, or stachyose as a carbon source. A highly significant correlation between dry weight and embryo number was obtained regardless of the carbohydrate source suggesting the involvement of a common intermediate in the metabolism of the various sugars.

In the presence of 2.25 µM 2,4-D, embryo formation was suppressed. Time course of dry weights obtained in the presence and absence of 2,4-D show that 2,4-D increased the growth rate of the tissue when glucose, fructose, mannose, or stachyose was used as the carbon source. The growth rates on other sugars remained unchanged under these conditions.

² A preliminary report of this work has appeared elsewhere (15).