Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 59:97-102 (1977)
© 1977 American Society of Plant Biologists

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Articles

Root Hair Cell Enhancement in Tissue Cultures from Soybean Roots: A Useful Model System

In Vitro Rhizobium Symbiosis 1,2

Narges Hermina and Minocher Reporter3

a Charles F. Kettering Research Laboratory, 150 East South College Street, Yellow Springs, Ohio 45387

A technique for obtaining large numbers of root hair cells in cell cultures from soybeans is described. The cells were grown on agar containing the Prairie Regional Laboratory B5 (PRL-B5) medium for periods longer than 60 days. Mixed populations of cultured root hair cells and cortical cells were used to study the in vitro association between soybean cells and Rhizobium japonicum. The advantages of these types of root cell cultures in studies of symbiosis are discussed.

3 To whom correspondence should be sent.

1 Some parts of this work were funded by National Science Foundation Grant BMS 75-17710.

2 This work is Contribution No. 578.






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Copyright © 1977 by the American Society of Plant Biologists