Plant Physiology 59:129-133 (1977)
© 1977 American Society of Plant Biologists
Articles
Tannins as Gibberellin Antagonists in the Synthesis of  -Amylase and Acid Phosphatase by Barley Seeds 1
Andrew Jacobson2 and
Mary Ritzel Corcoran3
a Department of Biology, California State University, Northridge, California 91324
The tannins chebulinic acid or tara tannin were added to an incubation system in which GA3 induces enzyme synthesis in endosperm half seeds of barley (Hordeum vulgare L.). The activity of amylase and acid phosphatase in the incubation medium was reduced compared to the activity in the medium after incubation with GA3 alone. When embryo half seeds of barley were incubated with chebulinic acid or tara tannin in the absence of added GA3, the enzyme activity of the incubation medium was also reduced. The activity of preformed enzymes obtained from endosperm half seeds previously induced with GA3 was not reduced by the addition of tannin. Comparisons were made of the amount of enzyme activity from breis of aleurone layers incubated with GA3 in the presence and absence of tannins. The amounts of activity were relatively small and approximately equal in both cases, indicating that secretion from the aleurone was not blocked by the tannins. The reduction of enzyme activity caused by tannins in both endosperm and embryo half seeds could be completely reversed by the addition of GA3.
2 Present address: Department of Soils and Plant Nutrition, University of California, Berkeley, Calif. 94709.
3 To whom reprint requests may be sent.
1 This investigation was supported in part by a fellowship from the California Foundation for Biochemical Research.
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