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Temperature-dependent Response to Indoleacetic Acid Is Altered by NH₄⁺ in Cultured Cotton Ovules ¹

^a Department of Plant Sciences, University of California, Riverside, California 92502

Unfertilized cotton ovules, cultured in vitro, produced fiber in response to indoleacetic acid (IAA) and/or gibberellic acid (GA₃). Of the two hormones, IAA produced the greater amount of fiber per ovule, and the effect of both hormones applied simultaneously was approximately additive. Depending upon in vitro culture temperature, a variable number of unfertilized cotton ovules produced fiber in response to 5 µM IAA. The response to IAA was "all or none" for each ovule. The percentage of ovules responding to 5 µM IAA ranged from zero at 28 C to 100 at 34 C. The ability to respond at nonpermissive culture temperatures (i.e. 28 to 30 C) was markedly increased by including 2.5 mM NH₄NO₃ in the KNO₃-based medium or by increasing concentrations of IAA. The inclusion of NH₄⁺ in the basal culture medium provided only an increased percentage of ovules producing fiber in the presence of IAA, but did not increase fiber production per ovule. Temperature, like GA₃, increased both the number of ovules producing fiber in response to IAA and the amount of fiber produced per ovule.

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