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Plant Physiology 59:365-368 (1977)
© 1977 American Society of Plant Biologists

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Articles

Effects of Osmotic Shock on Some Membrane-regulated Events of Oat Coleoptile Cells 1

Bernard Rubinsteina and Peter Mahara

Terry A. Tattarb

a Department of Botany, University of Massachusetts, Amherst, Massachusetts 01002, Department of Plant Pathology, University of Massachusetts, Amherst, Massachusetts 01002

Oat coleoptile sections (Avena sativa L. cv. "Garry") were osmotically shocked with 0.5 M mannitol followed by 1 mM Na-phosphate (pH 6.4) at 4 C. This treatment reduced uptake of {alpha}-aminoisobutyric acid, 3-o-methyl glucose, and leucine by 75 to 90% but inhibited 36Cl– uptake only 30%. Some recovery was observed 1 to 3 hours later. Respiration rates were unaffected by osmotic shock and protein synthesis was reduced 11%.

Osmotic shock also stimulated efflux of {alpha}-aminoisobutyric acid and K+ and led to an increase in conductivity of the solution bathing shocked sections. The transmembrane electropotential of 75% of the shocked cells fell to –20 mv to –45 mv compared with the majority of unshocked cells at –80 mv to –120 mv.

We concluded that osmotic shock selectively modifies the plasma membrane. The inhibitions of uptake could be due to removal of specific components of the plasma membrane and/or to the lowered electropotential.

1 Supported by Grant PCM 76-00439 from the National Science Foundation to B. R., a Grant-in-Aid of Research from the Society of Sigma Xi to P. M., and Massachusetts Agricultural Experiment Station Project Hatch 364.




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R. H. RACUSEN, A. M. KINNERSLEY, and A. W. GALSTON
Osmotically Induced Changes in Electrical Properties of Plant Protoplast Membranes
Science, October 28, 1977; 198(4315): 405 - 407.
[Abstract] [PDF]


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Copyright © 1977 by the American Society of Plant Biologists