Plant Physiol.
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Plant Physiology 59:525-529 (1977)
© 1977 American Society of Plant Biologists

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Articles

Storage Protein Synthesis in Maize

II. Reduced Synthesis of a Major Zein Component by the Opaque-2 Mutant of Maize 1,2

Richard A. Jones3, Brian A. Larkins and C. Y. Tsai

a Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907

Two zein proteins (Z1 and Z2) represent the majority of the protein synthesized during maize endosperm development. Undegraded membrane-bound polysomes isolated from normal maize synthesized these proteins when incubated in a cell-free protein-synthesizing system from wheat germ. The proteins synthesized in vitro were similar to authentic zein in ethanol solubility and electrophoretic mobility. Zein synthesis was associated with large size classes of membrane bound polysomes in normal maize.

Membrane-bound polysomes isolated from developing kernels of opaque-2 mutant synthesized less total zein in vitro, and dramatically reduced incorporation into the Z1 component. The reduction in total zein corresponded to a 50% reduction in the level of membrane-bound polysomes in opaque-2, and the near absence of the large polysome size classes, which synthesized zein in normal maize. We concluded that the opaque-2 mutation results in a decreased "availability" of the zein mRNAs, reflected in a reduced level of membrane-bound polysomes.

3 Present address: Department of Vegetable Crops, University of California, Davis, Calif. 95616.

1 This work was supported by a grant from the Lilly Endowment and a David Ross Grant to C. Y. T.

2 Journal Paper No. 6374 of the Purdue University Agricultural Experiment Station.






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Copyright © 1977 by the American Society of Plant Biologists