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Articles

Purification and Partial Characterization of a Lectin from Phaseolus vulgaris¹

^a Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, R.J., Brazil

A method is presented for the isolation of a lectin from a Brazilian cultivar of the common bean (Phaseolus vulgaris L.), through extraction in acidic (pH 4.2) medium, fractionation with ammonium sulfate, and chromatography on DEAE-cellulose. The lectin was shown to be homogeneous by gel electrophoresis and isoelectric focusing.

The molecular weight, determined by osmometry, is 100,250 daltons; the isoelectric point, determined by isoelectric focusing, is pH 5.1; and the extinction coefficient at 280 nm and pH 7 is E^1%_{1 cm} = 7.85.

The lectin was shown to be a glycoprotein with 9.11% of neutral sugars and 1.44% of amino sugars. Its amino acid composition was characterized by the absence of methionine and a very low content of half-cystine, with a predominance of acidic and hydroxylated amino acids.

The lectin presented no specificity when tested with red blood cells of all the groups of the ABO system.

¹ This work was part of the D.Sc. thesis of R. A. M., presented to the Dept. de Bioquímica do Instituto de Química da Universidade Federal do Rio de Janeiro, on March 14, 1975.

Part of the research was developed at the Dept. de Bioquímica do Instituto de Química da Universidade Federal do Rio de Janeiro and part at the Dept. de Bioquímica e Biologia Molecular do Centro de Ciências da Universidade Federal do Ceará. The research was supported by Coordenação do Aperfeiçoamento do Pessoal de Nível Superior with a grant to R. A. M., and by funds from OAS to the Dept. de Bioquímica e Biologia Molecular do Centro de Ciências da Universidade Federal do Ceará.

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