Plant Physiol.
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Plant Physiology 59:831-835 (1977)
© 1977 American Society of Plant Biologists

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Articles

Biochemical Responses of Pea Root Tissue to Cytokinin

Enhanced Rates of RNA Synthesis 1

Terry L. Shininger and Lowell D. Polley2

a Department of Biology University of Utah, Salt Lake City, Utah 84112

Rates of RNA synthesis were studied in cultured pea (Pisum sativum) root segments and cortical explants which require the hormone cytokinin for DNA replication and cell proliferation. Rate calculations were based on the specific radioactivity of the extracted RNA and the specific radioactivity of the extracted ATP pool after a pulse with 3H-adenosine. The kinetics of RNA synthesis was studied after 24 hours of culture with or without kinetin. We found that kinetin stimulated a 2- to 4-fold enhancement in the rate of RNA synthesis after 24 hours of culture as compared to controls. A similar order of magnitude of stimulation of RNA synthesis was found when RNA was isolated by cesium chloride centrifugation. Pulses during the first 24 hours indicate that kinetin stimulates the rate of RNA synthesis as early as 9 hours after treatment has begun. During the first 24 hours of culture, kinetin did not affect the specific radioactivity of the ATP pool. The ATP pool equilibrated slowly with the exogenous label (3H-adenosine) in the presence or absence of kinetin. After 3 days in culture, we found kinetin to cause an expansion of the extractable ATP pool and a corresponding reduction in the ATP pool specific radioactivity. We interpret these results to indicate a stimulation in the rate of RNA synthesis due to kinetin treatment prior to any other known response.

2 Present address: Department of Biology, Washington University, St. Louis, Mo.

1 This work was supported by National Institutes of Health Biomedical Research Support Grant RR 070902.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1977 by the American Society of Plant Biologists