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Blue light-induced Absorbance Changes in Membrane Fractions from Corn and Neurospora^1,2

^a Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305

Blue light-induced absorbance changes were measured from differentially centrifuged membrane fractions from dark-grown coleoptiles of Zea mays L., and mycelia from an albino mutant of Neurospora crassa. Actinic irradiation caused changes in absorbance consistent with a flavinmediated reduction of a b-type cytochrome. Both corn and Neurospora showed similar light-minus-dark difference spectra, dose response curves, and kinetics of dark recovery after irradiation. The photoreducible cytochrome system from Neurospora showed the same distribution as the activity of a sodium-stimulated adenosine triphosphatase, thought to be a plasma membrane marker, in differential centrifugation experiments. The fraction showing the absorbance change did not co-sediment with the mitochondria, nor with the endoplasmic reticulum. Comparison of absorption spectra of fully oxidized, partially reduced, and fully reduced preparations showed that approximately a 30% reduction of the cytochromes involved with the process was needed to obtain the light-induced absorbance changes.

⁴ Present address: Dept. of Biology, Menlo School, Atherton, Calif. 94025.

¹ This work has been supported by National Science Foundation Undergraduate Research Award No. EPP75-04559 to R. D. B. and Carnegie Institution of Washington, Dept. of Plant Biology, Stanford, Calif. 94305.

² CIW-DPB Publication No. 578.

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